The Incorporation and Metabolism of Glucose by Anabaena variabilis

The metabolism of acetate by the blue-green algae, Anabaena variabilis and Anacystis nidulans.

The utilization of acetate by blue-green algae was examined and the activities of enzymes involved in its metabolism measured. Although acetate did not stimulate the endogenous respiration of these organisms, the oxidation of acetate was followed by the rate of release of [14C] carbon dioxide from [I-~~CC] and [2-l4CC] sodium acetate. Similarly, sodium acetate did not alter the rate of growth o...

Bioenergetics Studies of the Cyanobacterium Anabaena variabilis

A cell-free system exhibiting both photophosphorylation (P /2e = 1) and oxidative phosphoryla­ tion (P/O up to 0 .8 ) is described for the cyanobacterium Anabaena variabilis. NADH and N A D PH were found to be equally effective as electron donors for oxidative phosphorylation. Low concentrations of U H D B T , an inhibitor of the cytochrome blc complex of mitochondria and chloroplasts. were fou...

Components of the proton electrochemical potential gradient in Anabaena variabilis.

RNA processing of nitrogenase transcripts in the cyanobacterium Anabaena variabilis.

Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied by using a lacZ reporter. Despite evidence for a transcription start site just upstream of nifH1 and vnfH, promoter fragments that included ...

Transcription of hupSL in Anabaena variabilis ATCC 29413 is regulated by NtcA and not by hydrogen.

Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PCC 7120 because A. variabilis lacks the excision element-mediated regulation th...